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Hydrogen Peroxide |
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 Methods
Hydrogen peroxide is a strong oxidizing agent with a variety of uses. Applications include the treating of industrial effluents and domestic waste and serving as a disinfectant in aseptic packaging.
The Ferric Thiocyanate Method
Reference: D. F. Boltz and J. A. Howell, eds., Colorimetric Determination of Nonmetals, 2nd. ed., Vol. 8, p. 304 (1978).
The ferric thiocyanate method consists of ammonium thiocyanate and ferrous iron in acid solution. Hydrogen peroxide oxidizes ferrous iron to the ferric state, resulting in the formation of a red thiocyanate complex. Chlorine will not interfere with this method. Ferric iron will interfere. Results are expressed as ppm (mg/L) H2O2.
The DDPD Method
Reference: Developed by CHEMetrics, Inc.
With the DDPD Method, hydrogen peroxide reacts with DDPD in the presence of ammonium molybdate to form a purple product. Results are expressed as ppm (mg/L) H2O2.
The Ceric Sulfate Titrimetric Method
Reference: Developed by CHEMetrics, Inc.
CHEMetrics developed a titrimetric method using ceric sulfate as the titrant and ferroin as the end point indicator. A color change from green to orange signals the end of the titration. Results are expressed as percent (%) H2O2.
The Ceric Sulfate Go-No-Go Method, NEW!
Reference: Developed by CHEMetrics, Inc.
Developed for clinical applications where hydrogen peroxide in sterilizing/disinfecting solutions must be monitored continuously for efficacy. A single, small dose of sample is added to a screw cap vial containing ceric sulfate and the endpoint indicator ferroin. An immediate color change occurs to signal that the hydrogen peroxide level in the sample is either above or below 6.0%. |
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| • Click to View MSDS and Kit instructions |
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 Visual Kits |
0-0.50 ppm
0.025 ppm / Method: DDPD |
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Cat# |
| CHEMets Kit |
K-5504 |
| | CHEMets Refill, 30 ampoules | R-5504 |
| | Activator Solution Pack, six 10 mL bottles, Shelf-life 2 years | A-2500 |
| | Activator Solution Pack, six 10 mL bottles, Shelf-life 2 years | A-5500 |
| | Comparator, Shelf-life 2 years:
0, 0.05, 0.10, 0.15, 0.20, 0.25, 0.30, 0.50 ppm | C-5504 |
| | Kit comes in a plastic case and contains everything needed to perform 30 tests: Refill, Comparator, Activator Solutions, 25 mL sample cup, instructions, and MSDS. | |
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0-1 & 1-10 ppm
0.05 ppm /Method: Ferric Thiocyanate |
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Cat# |
| CHEMets Kit |
K-5510 |
| | CHEMets Refill, 30 ampoules | R-5510 |
| | Low Range Comparator, Shelf-life 2 years:
0, 0.1, 0.2, 0.3, 0.4, 0.6, 0.8, 1.0 ppm | C-5501 |
| | High Range Comparator, Shelf-life 2 years:
1, 2, 3, 4, 5, 6, 7, 8, 10 ppm | C-5510 |
| | Kit comes in a plastic case and contains everything needed to perform 30 tests: Refill, Low and High Range Comparators, 25 mL sample cup, instructions, and MSDS. | |
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0-30 & 30-300 ppm
5 ppm / Method: Ferric Thiocyanate |
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Cat# |
| VACUettes Kit |
K-5510D |
| | VACUettes Refill, 30 ampoules | R-5510D |
| | Low Range Comparator, Shelf-life 2 years:
0, 5, 7.5, 10, 15, 20, 25, 30 ppm | C-5501D |
| | High Range Comparator, Shelf-life 2 years:
30, 60, 90, 120, 150, 175, 200, 250, 300 ppm | C-5510D |
| | Kit comes in a plastic case and contains everything needed to perform 30 tests (except distilled water): Refill, Low and High Range Comparators, dilutor snapper cup, micro test tube, instructions, and MSDS. | |
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0-60 & 60-600 ppm
10 ppm / Method: Ferric Thiocyanate |
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Cat# |
| VACUettes Kit |
K-5510A |
| | VACUettes Refill, 30 ampoules | R-5510A |
| | Low Range Comparator, Shelf-life 2 years:
0, 10, 15, 20, 30, 40, 50, 60 ppm | C-5501A |
| | High Range Comparator, Shelf-life 2 years:
60, 120, 180, 240, 300, 350, 400, 500, 600 ppm | C-5510A |
| | Kit comes in a plastic case and contains everything needed to perform 30 tests (except distilled water): Refill, Low and High Range Comparators, dilutor snapper cup, micro test tube, instructions, and MSDS. | |
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0-120 & 120-1200 ppm
20 ppm / Method: Ferric Thiocyanate |
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Cat# |
| VACUettes Kit |
K-5510B |
| | VACUettes Refill, 30 ampoules | R-5510B |
| | Low Range Comparator, Shelf-life 2 years:
0, 20, 30, 40, 60, 80, 100, 120 ppm | C-5501B |
| | High Range Comparator, Shelf-life 2 years:
120, 240, 360, 480, 600, 700, 800, 1000, 1200 ppm | C-5510B |
| | Kit comes in a plastic case and contains everything needed to perform 30 tests (except distilled water): Refill, Low and High Range Comparators, dilutor snapper cup, micro test tube, instructions, and MSDS. | |
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0-1200 & 1200-12,000 ppm
200 ppm / Method: Ferric Thiocyanate |
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Cat# |
| VACUettes Kit |
K-5510C |
| | VACUettes Refill, 30 ampoules | R-5510C |
| | Low Range Comparator, Shelf-life 2 years:
0, 200, 300, 400, 600, 800, 1000, 1200 ppm | C-5501C |
| | High Range Comparator, Shelf-life 2 years:
1200, 2400, 3600, 4800, 6000, 7000, 8000, 10,000, 12,000 ppm | C-5510C |
| | Kit comes in a plastic case and contains everything needed to perform 30 tests (except distilled water): Refill, Low and High Range Comparators, dilutor snapper cup, micro test tube, instructions, and MSDS | |
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2-20%
2.0% Method: Ceric Sulfate Titrant with Ferroin Indicator |
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Cat# |
| Titrets Kit |
K-5530 |
| | Increments:
2.0, 2.2, 2.4, 2.6, 2.8, 3.0, 3.2, 3.6, 4.0, 5.0, 6.0, 7.0, 8.0, 10, 14, 20% | |
| | Kit comes in a cardboard box and contains everything needed to perform 30 tests (except distilled water): thirty ampoules, thirty valve assemblies, dropper, titrettor, 50 mL sample cup, instructions, and MSDS. | |
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6.0% Control Point
Ceric Sulfate Titrant with Ferroin Indicator |
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Cat# |
| Go-No-Go Kit, NEW! |
K-5500C |
| | Kit comes in a cardboard box and contains everything needed to perform 30 tests: thirty 16-mm vials with caps, thirty pipettor tips, 100 uL pipettor, Indicator Solution, instructions, and MSDS. | |
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 Instrumental Kits |
0.20-2.00 ppm
DDPD |
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Cat# |
| Vacu-vials Kit |
K-5503 |
| | Kit comes in a cardboard box and contains everything needed to perform 30 tests: thirty ampoules, Activator Solutions, 25 mL sample cup, ampoule blank, instructions, calibration table, and MSDS. | |
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V-2000: 0.15-6.00 ppm /Spec: 0.15-4.00 ppm
Ferric Thiocyanate |
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Cat# |
| Vacu-vials Kit |
K-5543 |
| | Kit comes in a cardboard box and contains everything needed to perform 30 tests: thirty ampoules, 25 mL sample cup, ampoule blank, instructions, calibration table, and MSDS. | |
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0.20-2.00 ppm
DDPD |
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Cat# |
| VVR Module |
V-5503 |
| | Vacu-vials Kit, 30 ampoules, Activator Solutions, 25 mL sample cup, ampoule blank, instructions, calibration table, and MSDS. | K-5503 |
| | VVR Filter, Shelf-life 2 years | F-5503 |
| | Module comes in a cardboard box and contains everything needed to perform 30 tests: Vacu-vials Kit, VVR Filter, and instructions. | |
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0.15-4.00 ppm
Ferric Thiocyanate |
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Cat# |
| VVR Module |
V-5543 |
| | Vacu-vials Kit, 30 ampoules, 25 mL sample cup, ampoule blank, instructions, calibration table, and MSDS. | K-5543 |
| | VVR Filter, Shelf-life 2 years | F-5543 |
| | Module comes in a cardboard box and contains everything needed to perform 30 tests: Vacu-vials Kit, VVR Filter, and instructions. | |
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0.15-6.00 ppm
Ferric Thiocyanate |
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Cat# |
| Hydrogen Peroxide SAM Kit, NEW! |
I-2016 |
| | Vacu-vials Kit, 30 ampoules, 25 mL sample cup, ampoule blank, instructions, calibration table, and MSDS. | K-5543 |
| | SAM Kit comes in a plastic case and contains everything needed to perform 30 tests: Vacu-vials Kit, SAM Photometer, 2 AA batteries, and instructions. | |
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Kit Components common to Hydrogen Peroxide |
| Description |
Cat# |
| | Sample Cup Pack, 25 mL (6 ea) | A-0013 |
| | Mico Test Tube Pack (10 ea) | A-0015 |
| | Dilutor Snapper Cup Pack (6 ea) | A-0018 |
| | Ampoule Blank Pack (5 ea) | A-0023 |
| | Dropper Pack, 0.25 mL (10 ea) | A-0029 |
| | Titrettor Pack (1 ea) | A-0053 |
| | Sample Cup & Cap Pack, 50 mL (6 ea) | A-0058 |
| | Pipettor Pack, 100 mL (1 ea) | A-0170 |
| | Pipettor Tips Pack, (30 ea) | A-0171 |
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